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[qPCR/Electrophoresis]dsGreen Gel Staining Solution, 10000×

dsGreen Gel Staining Solution, 10000×

訂購貨號(hào)產(chǎn)品名稱及規(guī)格價(jià)格(￥)

20010dsGreen Gel Staining Solution, 10000×, 1 mL2340.00

SYBR Green I是一種敏感的dsDNA結(jié)合染料，可以用于日常瓊脂糖和聚丙烯酰胺凝膠的DNA檢測(cè)。

不同于Ethidium，SYBR Green I對(duì)雙鏈DNA有高度選擇性，更低的毒性，更高的靈敏度。

溴化乙錠(Ethidium Bromide)和SYBR Green I的對(duì)比

FeatureEthidium BromideSYBR Green I

FluorescenceRed (615 nm)Green (524 nm)

Excitation maximum302 nm454 nm

Excitation light sourceUV onlyBlue light or UV

Sensitivity2 ng / band(dsDNA)

100 ng / band (RNA)0.08 ng / band (dsDNA)

1-2 ng / band (oligonucleotides)

Health hazardHighLow

推薦手冊(cè)：

SYBR Green I DNA凝膠染色

Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

General properties

Appearance: orange solution

Quality control: NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

MSDS: Download

Spectral properties

Excitation maximum, nm: 454

Extinction coefficient at excitation maximum, Lmol-1cm-1: 73000

Emission maximum, nm: 524

Fluorescence quantum yield: 0.8

[qPCR/Electrophoresis]dsGreen Gel Staining Solution, 10000×新品發(fā)布，歡迎咨詢選購。