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Cyanine7 azide

ROX reference dye for qPCR

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31110ROX reference dye for qPCR, 500 uL420.00

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as SYBR Green I or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.

The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween?-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

General properties

Appearance: pink solution

Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Spectral properties

Excitation maximum, nm: 570

Emission maximum, nm: 591

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