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Pentynoic acid STP ester

Pyrene phosphoramidite dU

訂購貨號產(chǎn)品名稱及規(guī)格價格(￥)

11460Pyrene phosphoramidite dU, 100 mg1950.00

21460Pyrene phosphoramidite dU, 250 mg4160.00

41460Pyrene phosphoramidite dU, 1 g11440.00

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

General properties

Appearance: yellowish foam

Molecular weight: 955.04

Molecular formula: C57H55N4O8P

Solubility: good in dichloromethane and acetonitrile

Quality control: NMR 1H (95%) and 13C, TLC, HPLC

Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS: Download

Spectral properties

Excitation maximum, nm: 260; 282; 365; 392

Extinction coefficient at excitation maximum, Lmol-1cm-1: 12600; 21900; 16000; 14200

Emission maximum, nm: 460

Fluorescence quantum yield: ~0.1

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